#Flaxseed allergy cross reactivity skin
Skin prick test (prick by prick) with linseed was positive, with an immediate response wheal of 5x4 mm in diameter. The maximal release was induced by 10 % perchlorate (Sigma, St Louis, MO, USA). Purified horse anti-human IgE (Tago, Burlingame, CA, USA) was used as a positive control. LHRT was performed as described 7 in order to indirectly detect specific serum IgE antibodies against our linseed extract. The protein concentration, as determined by Bradford assay 6 (Bio-Rad, Munchen, Germany) was 3.6 mg/ml.īoth were measured by CAP enzymo-immunoassay (Pharmacia Diagnostics AB, Uppsala, Sweden). After stirring and spinning, the supernatant was passed through a Millipore filter (0.22 () for sterilization (Millipore, Molsheim, France). Two grams of linseeds were defatted in acetone and extracted in 20 ml of phosphate buffered saline (PBS) at room temperature (RT).
Prick by Prick tests were also performed with linseed. She had multisystemic involvement (abdominal pain, vomiting, urticaria, dyspnea, dysphonia, dysphagia and hypotension) and fully recovered after being treated at the emergency room.Ī battery of commercially available common food allergens (Bial-Aristegui, Bilbao, Spain) was tested with the allergy pricker lancet (Dome-Hollister-Stier) as described 5. This reduction in MW after the destruction of disulphide bonds suggested a multimeric protein as the causative antigen.Ī 39 year-old woman, with a previous history of hay fever and intolerance to egg, suffered an anaphylactic reaction immediately after the ingestion of the first spoonful of grains of linseed, prescribed as a laxative. In the former report 3, weak to mild IgE reactivity to multiple bands in the immunoblotting did not allow for the identification of the involved allergen(s), but it was noted by the authors that the weight of these bands was diminished (from 150-175 to 35-100 kDa) after SH 2 reduction of the sample. Anaphylaxis induced by flax has also been described, and the allergens of both grain 3 (in multigrain bread) and oil 4 (as a laxative) have been analyzed. Hypersensitivity to linseeds, though infrequent, has been known for long 1.In our time, linseed oil is increasingly used as a laxative 2. Linum usitatissimum (flax) is a plant belonging to the Linaceae family which is used in the textile industry. Letters to the Editor on previously published papers are welcomed.Allergologia et Immunopathologia publishes 6 issues per year and is included in the major databases such as Pubmed, Scopus, Web of Knowledge, etc. Occasionally, the opinion of an expert on a burning topic is published in the "Point of View" The journal accepts original and review articles from all over the world, together with consensus statements from the aforementioned societies.
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Allergologia et Immunopathologia is the official journal of the Spanish Society of Pediatric Allergy and Clinical Immunology. Manuscripts related to clinical, epidemiological and experimental allergology and childhood immunopathology will be considered for publication. Allergologia et Immunopathologia is no longer published on Elsevier since the 2021 year.Transferred to Codon PublicationsĪllergologia et Immunopathologia is a forum for those working in the field of asthma, allergy and immunology related to childhood.
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